High-throughput analysis of peptide-binding modules
نویسندگان
چکیده
منابع مشابه
High-throughput engineering and analysis of peptide binding to class II MHC.
Class II major histocompatibility complex (MHC-II) proteins govern stimulation of adaptive immunity by presenting antigenic peptides to CD4+ T lymphocytes. Many allelic variants of MHC-II exist with implications in peptide presentation and immunity; thus, high-throughput experimental tools for rapid and quantitative analysis of peptide binding to MHC-II are needed. Here, we present an expressio...
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Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or design(1,2). Here, a peptide-MHC II binding assay is scaled to 384-well format. The scaled down protocol reduces reagent costs by 75% and is higher throughput than previously described 96-well protocols(1,3-5). Specifically, the experimental d...
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15 صفحه اولIdentification of target-binding peptide motifs by high-throughput sequencing of phage-selected peptides
High-throughput sequencing was previously applied to phage-selected peptides in order to gain insight into the abundance and diversity of isolated peptides. Herein we developed a procedure to efficiently compare the sequences of large numbers of phage-selected peptides for the purpose of identifying target-binding peptide motifs. We applied the procedure to analyze bicyclic peptides isolated ag...
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ژورنال
عنوان ژورنال: PROTEOMICS
سال: 2012
ISSN: 1615-9853
DOI: 10.1002/pmic.201100599